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X-ray crystallography; Cryoelectron microscopy; 8-angstrom resolution; Atomic model; Virus; Crystallization; Visualization; Channel; Reconstruction; Tomography.; Biochemistry & Biophysics in Current Contents(R)/Life Sciences.
Abstract:
Macromolecular machines carry out many cellular functions. Cryo-electron microscopy (cryo-EM) is emerging as a powerful method for studying the structure, assembly and dynamics of such macromolecules, and their interactions with substrates. With resolutions still improving, 'single-particle' analyses are already depicting secondary structure. Moreover, cryo-EM can be combined in several ways with X-ray diffraction to enhance the resolution of cryo-EM and the applicability of crystallography. Electron tomography holds promise for visualizing machines at work inside cells. [References: 62]
