ausblenden:
Sprache(n):
eng - English
Datum:
2010-01-31
Publikationsstatus:
Angenommen
Seiten:
62 Bl.
Ort, Verlag, Ausgabe:
Bingen : Fachhochschule
Inhaltsverzeichnis:
Table of contents
List of Abbreviations...................................................................................................5
List of figures..............................................................................................................6
List of tables...............................................................................................................7
Acknowledgments......................................................................................................8
1. Introduction.............................................................................................................9
1.1 Natural selection and adaptations.....................................................................9
1.2 The worldwide migration of Mus musculus domesticus..................................12
1.3 Copy number variants.....................................................................................14
1.4 Molecular signature of recent positive selection..............................................16
1.5 Microsatellites as markers of genetic variability..............................................17
1.6 Goal and overview of the study.......................................................................19
2. Materials and methods.........................................................................................20
2.1 Materials.........................................................................................................20
2.1.1 Lab equipment..........................................................................................20
2.1.2 Chemicals.................................................................................................21
2.1.3 Programmes.............................................................................................22
2.1.4 Animals.....................................................................................................23
2.2 Methods..........................................................................................................24
2.2.1 DNA isolation and purification..................................................................24
2.2.2 Polymerase chain reaction (PCR)............................................................24
2.2.3 Agarose gel electrophoresis.....................................................................25
2.2.4 Primer design for qPCR............................................................................26
2.2.5 Microsatellite PCR and sequencing..........................................................26
3. Results..................................................................................................................28
3.1 Design of the primers to amplify putative copy-number variable regions........28
3.1 Primer efficiency test.......................................................................................30
3.2 Quantitative PCR............................................................................................34
3.4 Microsatellite scan for selective sweeps.........................................................38
4. Discussion...........................................................................................................41
4.1. Technical issues concerning quantitative PCR assays..................................41
4.2. Results of the quantitative PCR assays.........................................................42
4.3. Results of the microsatellite screen................................................................43
4.4. Future work........................................................................................................44
6. References...........................................................................................................45
7. Attachment...........................................................................................................52
Art der Begutachtung:
-
Identifikatoren:
eDoc: 453067
Anderer: Diss/12200
Art des Abschluß:
Diplom
