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  Controlling the Selectivity and Stability of Proteins by New Strategies in Directed Evolution: The Case of Organocatalytic Enzymes

Reetz, M. T. (2008). Controlling the Selectivity and Stability of Proteins by New Strategies in Directed Evolution: The Case of Organocatalytic Enzymes. In M. T. Reetz, B. List, S. Jaroch, & H. Weinmann (Eds.), Organocatalysis. Ernst Schering Foundation Symposium Proceedings, Vol. 2007/2 (pp. 321-340). Berlin Heidelberg: Springer-Verlag. doi:10.1007/2789_2007_068.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-90F1-9 Version Permalink: https://hdl.handle.net/21.11116/0000-0008-47DD-0
Genre: Book Chapter

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 Creators:
Reetz, Manfred T.1, Author           
Affiliations:
1Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445588              

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 Abstract: The directed evolution of functional enzymes as catalysts in organic reactions has emerged as a powerful method of protein engineering. This includes the directed evolution of enantioselective enzymes as pioneered by the author. In recent years the challenges in this new area of asymmetric catalysis has shifted to solving the problem of probing protein sequence space more efficiently than before. Iterative saturation mutagenesis (ISM) is one way of addressing this crucial question. This chapter reviews the concept of ISM and its application in controlling the enantioselectivity and thermostability of enzymes, specifically those that have an organocatalytic mechanism. Illustrative examples include the directed evolution of lipases, Baeyer-Villigerases and epoxide hydrolases.

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Language(s): eng - English
 Dates: 2007-12-182008
 Publication Status: Issued
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/2789_2007_068
 Degree: -

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Title: Organocatalysis. Ernst Schering Foundation Symposium Proceedings, Vol. 2007/2
Source Genre: Series
 Creator(s):
Reetz, Manfred T.1, Editor           
List, Benjamin2, Editor           
Jaroch, Stefan3, Editor
Weinmann, Hilmar3, Editor
Affiliations:
1 Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445588            
2 Research Department List, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445585            
3 Bayer Schering Pharma AG, 13342 Berlin, Germany, ou_persistent22            
Publ. Info: Berlin Heidelberg : Springer-Verlag
Pages: - Volume / Issue: 2007/2 Sequence Number: - Start / End Page: 321 - 340 Identifier: ISBN: 9783540734949