Flavoproteins in Directed Evolution

Kille, Sabrina

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Flavoproteins in Directed Evolution

Kille, S. (2010). Flavoproteins in Directed Evolution. PhD Thesis, Ruhr-Universität Bochum, Bochum.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-000F-8D0D-4 Versions-Permalink: https://hdl.handle.net/21.11116/0000-000D-70E5-2

Genre: Hochschulschrift

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Urheber:
Kille, Sabrina¹, Autor

Affiliations:
1Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445588

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Zusammenfassung: Two enzymes, an enoate reductase (YqjM) and a monooxygenase (P450_BM3), were
successfully altered towards acceptance of new substrates applying directed evolution. Using iterative CASTing (combinatorial active site saturation test) highly regio- and stereoselective mutants were obtained in both cases. Additionally it was possible to control the regioselectivity (P450_BM3) and enantioselectivity (YqjM) of the catalyzed reaction.
Moreover this work was used to shed more light on the iterative CASTing methodology and its application and the gained insights contribute to a more efficient application of the method.